Regulatory

Part:BBa_K2737004:Design

Designed by: Xinyu Teng   Group: iGEM18_ECUST   (2018-10-06)


constitutive promoter with fur box downstream -10 region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Fur box is between -10 region and RBS.


Source

Promoter of lacI from plasmid pET28a+. Sequence downstream -10 region is replaced with fur box gataatgattatca

References